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Anti Filaggrin Rabbit Polyclonal, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
Anti Filaggrin Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
Filaggrin Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
Anti Filaggrin (Flg) Mouse Monoclonal Antibody Gtx23137, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
Anti Filaggrin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of <t>filaggrin</t> protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.
Anti Filaggrin, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 6 Effect of PNs on expression of filaggrin protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.

Journal: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

Article Title: Polynucleotides Enhance Skin Barrier Function and Reduce Inflammation in a 2,4-Dinitrochlorobenzene-Induced Mouse Model of Atopic Dermatitis.

doi: 10.1111/srt.70189

Figure Lengend Snippet: FIGURE 6 Effect of PNs on expression of filaggrin protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.

Article Snippet: Skin sections were immunostained with an anti-filaggrin antibody (1: 500, orb10662; Biorbyt, Durham, NC, USA) according to the manufacturer’s guidelines.

Techniques: Expressing, Staining, Western Blot, Control, Saline

FIGURE 6 Effect of PNs on expression of filaggrin protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.

Journal: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

Article Title: Polynucleotides Enhance Skin Barrier Function and Reduce Inflammation in a 2,4-Dinitrochlorobenzene-Induced Mouse Model of Atopic Dermatitis.

doi: 10.1111/srt.70189

Figure Lengend Snippet: FIGURE 6 Effect of PNs on expression of filaggrin protein in a DNCB-induced AD model. Skin sections were prepared from dorsal skin harvested on Day 30 and immunohistochemically stained with an anti-filaggrin antibody. (A) Images were taken at 40× magnification. (B) The density of filaggrin staining in dorsal skin was quantified as the mean of five randomly selected fields per section and analyzed using ImageJ. Data are presented as the mean ± SD (n = 6). (C) Filaggrin protein expression levels in dorsal skin tissue lysates were evaluated using Western blot analysis. (D) Densitometric analysis of the Western blot bands was performed to quantify filaggrin protein expression levels. * p < 0.05 versus normal group. # p < 0.05 versus control group. $ p < 0.05 PN group versus PN+HA group. Normal: unstimulated group; control: DNCB-stimulated and saline-treated group; PN: DNCB-stimulated and PN-treated group; HA: DNCB-stimulated and HA-treated group; PN+HA: DNCB-stimulated and PN+HA-treated group; DEX: DNCB-stimulated and 0.01% dexamethasone-treated group.

Article Snippet: Equal amounts of protein were separated via SDS-PAGE, transferred onto polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA), and probed with a filaggrin antibody (1:500, orb10662, Biorbyt).

Techniques: Expressing, Staining, Western Blot, Control, Saline